Dexamethasone blocks motor impairment and brain morphological changes induced by the mixture of taurine and alcohol in a mice model of alcohol hangover

PINTO, A.; CUTRERA RA; CARBONE S; GOLDSTEIN, J.

Mar del Plata

Congreso; LXIV REUNION ANUAL SOCIEDAD ARGENTINA DE INVESTIGACION CLINICA; 2019

Sociedad Argentina de Investigacion Clinica

Abstract/Resumen: It has been proposed that inflammatory mechanisms could be involved in alcohol hangover (AH). In previous work we demonstrated that at the beginning of AH micetreated with alcohol (OH) and taurine (TAU), the main component of energy drinks, showed behavioral and morphological changes in the brain. The aim of this work was to study if the pretreatment with dexamethasone (DEXA) could block the motor disabilities and the neural stress produced by TAU in an experimental model of AH. Mice (n= 8-12) were ip pretreated with DEXA (7.5 mg/kg) 24 h before the i.p. injection of OH (3.8 mg/kg) and/or TAU (190 mg/kg). Controls were injected with saline. The Tight rope (TR) and Hanging wire (HW) tests were used to study neuromuscular coordination and muscle tension, respectively. Another group of mice (n= 8) wereperfused and the brains were subjected to immunofluorescence with an anti-GFAP antibody (glial fibrillary acidic protein) and an anti-MBP antibody (myelin basic protein) to identify reactiveastrocytes and myelin sheath respectively. All the studies were performed 6 h after treatment with OH and/or TAU (beginning of AH). It was observed that DEXA blocked significantly (ANOVA-Tukey, p