Osmolar stress altered the differentiation of extravillous trophoblast pathway.

ANUD C.; MARTÍNEZ N.; REPPETTI J.; DAMIANO A. E.; SIERRA M.; FARINA M.

Ciudad Autonoma de Buenos Aires

Congreso; IFPA 2019- VIII SLIMP; 2019

International Federation of Placenta Associations

OSMOLAR STRESS ALTERED THE DIFFERENTIATION OF EXTRAVILLOUSTROPHOBLAST PATHWAYMatías N. Serra 1, Julieta Reppetti 2, Carolina Anud 1, Mariana Farina 3, AliciaE. Damiano 1,2, Nora Martínez 2. 1 Catedra de Biología Celular y Molecular.Departamento de Ciencias Biologicas, Facultad de Farmacia y Bioquímica,Buenos Aires, Argentina; 2 Instituto de Fisiología y Biofísica BernardoHoussay (IFIBIO Houssay) UBA-CONICET, Buenos Aires, Argentina; 3 Centrode Estudios Farmacologicos y Botanicos (CEFyBO) UBA-CONICET, BuenosAires, ArgentinaObjectives: To explore the effect of hyperosmolarity on the extravilloustrophoblast cells migration, invasion and endovascular differentiation.Methods: Swan-71 cell line (human extravillous trophoblast cells) wascultured in complete DMEM-F12 and different concentrations of sucrosewere added to generate the osmolar stress.Cell migration was evaluated by wound healing assay, activity of metalloproteinases(MMPs) by zymography, cell invasion was evaluated intranswells pre-coated with Matrigel® and endovascular differentiationwas analyzed by the formation of tube-like structures in plates coated withMatrigel®.Results: In all the conditions tested cell viability was not modified.Hyperosmolarity significantly reduced cell migration, MMPs activity and cell invasion in Swan-71 cells (33 ± 4%, 75 ± 8%, and 45 ± 6%, respectivelycompared to iso-osmolar condition; p