IFIBIO HOUSSAY   25014
INSTITUTO DE FISIOLOGIA Y BIOFISICA BERNARDO HOUSSAY
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
THE LOSS OF FUNCTIONALITY OF AQUAPORIN 9 ADVERSELY AFFECTS THE SURVIVAL OF TROPHOBLAST CELLS
Autor/es:
MEDINA, YOLLYSETH; DOROSSHNKO BRIAN; ANUD CAROLINA; DAMIANO, ALICIA E.; DI PAOLA, MAURICIO; REPPETTI, JULIETA
Lugar:
Rosario, Argentina
Reunión:
Congreso; SAFIS 2019; 2019
Institución organizadora:
Sociedad Argentina de Fisiología
Resumen:
Trophoblast apoptosis is a physiological process during placentation and increases progressively throughout gestation. It is notably exaggerated inpregnancy complications such as preeclampsia. We have previously reported that aquaporin 9 (AQP9) protein expression increased in preeclamptic placentas buy it was not functional. Recently, we demonstrated that the exacerbated oxidative and nitrative stress may induce the nitration of AQP9 resulting in a non-functional protein. In addition to water, AQP9 can permeate small uncharged solutes such as lactate. Lactate can be used as an energy substrate to prevent cell death.Objective: Our aim was to explore the role of AQP9 in the survival of trophoblast cells. Methodology: This study was approved by the ethics committee of the Hospital Nacional Dr. Prof. A. Posadas. Explants from normal term placentas were cultured in low glucose conditions with or without L-lactate, and in presence and absence of AQP9 inhibitors (0.3 mM HgCl2, a general blocker of AQPs, and 0.5mM Phloretin, to block AQP9). Bax/Bcl-2 protein expression ratio by Western blot,cell viability by MTT assay and cytotoxicity by LDH release were assessed.Results: In explants cultured in low glucose medium, MTT decreased and Bax/Bcl-2 ratio increased compared to controls, showing that cells were dying (n=5, p