Detection of Shiga toxin type 2 binds to microvesicles in the plasma simple of a patient with Shigellosis.

GOMEZ FERNANDO D; SACERDOTI FLAVIA; ZOTTA ELSA; ISERN ELSA; AMARAL MARÍA MARTA; PROPORATO MELINA; PELLEGRINI MARIANA; IBARRA CRISTINA

Mar del Plata

Congreso; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica.; 2019

Sociedad Argentina de Investigación Clínica

Shigatoxin type 2 (Stx2) are mainly produced by Shiga toxin?producing Escherichia coli (STEC) that it has beenreported to be highly pathogenic and to be associated with hemorrhagic colitis hemolyticand uremic syndrome (HUS). Atthis moment, an early method of diagnosis of HUS is not exist. Our laboratorydeveloped a method of detection of Stx2 bind to microvesicles.Theaim was to detect plasma Stx2 bounded to microvesicles (MVs-Stx2) in a patientwith Shigellosis and systemic complications. A 10-year-old girl was admitted to the hospital because of mucousdiarrhea with bloody stretch marks overtwo days. Her laboratory admissionshowed normal hematocrit, hemoglobin and platelet count.  Renal functionwas conserved without hematuria and proteinuria. Coproculture sample waspositive for Shigella flexneri. Two days after admission,hematocrit (30.7%), hemoglobin (9.8 g/dl) and platelet count (187,000/mm3) were decreased.Take into account this result it was suspected a development of HUS. Aperipheral blood smear was performed and did not show schistocytosis. Weobtained blood samples in order to detect the presence of plasma Stx2. Samples were sequentiallyultracentrifuged to obtain microvesicles (MVs)-enriched suspension. Then, MVscarrying Stx2 were analyzed by flow cytometry. Data are expressed as the percentageof positives MVs-Stx2. From the controls, a cut-off range for MVs-Stx2 wasestablished (1.02-1.90 %, n = 5). A significant higher percentage of MVs-Stx2(13.6 %) was detected. The patient was not present renal function alterationsduring this time.Theseresults indicate that the systemic alterations observed in this patient couldbe explain by the effects of circulating Stx2, like happens in patients withincomplete HUS (absence of kidney disorders) Based on this, it is essential toincorporate Stx2 detection to patients with bacterial infection such asShigellosis.<!-- /* Font Definitions */ @font-face{font-family:"MS Mincho";panose-1:2 2 6 9 4 2 5 8 3 4;mso-font-alt:"ＭＳ 明朝";mso-font-charset:128;mso-generic-font-family:modern;mso-font-pitch:fixed;mso-font-signature:-536870145 1791491579 134217746 0 131231 0;}@font-face{font-family:"Cambria Math";panose-1:2 4 5 3 5 4 6 3 2 4;mso-font-charset:0;mso-generic-font-family:roman;mso-font-pitch:variable;mso-font-signature:-536869121 1107305727 33554432 0 415 0;}@font-face{font-family:Calibri;panose-1:2 15 5 2 2 2 4 3 2 4;mso-font-charset:0;mso-generic-font-family:swiss;mso-font-pitch:variable;mso-font-signature:-469750017 -1073732485 9 0 511 0;}@font-face{font-family:"\@MS Mincho";panose-1:2 2 6 9 4 2 5 8 3 4;mso-font-charset:128;mso-generic-font-family:modern;mso-font-pitch:fixed;mso-font-signature:-536870145 1791491579 134217746 0 131231 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal{mso-style-unhide:no;mso-style-qformat:yes;mso-style-parent:"";margin:0cm;margin-bottom:.0001pt;mso-pagination:widow-orphan;font-size:12.0pt;font-family:"Calibri",sans-serif;mso-ascii-font-family:Calibri;mso-ascii-theme-font:minor-latin;mso-fareast-font-family:"MS Mincho";mso-fareast-theme-font:minor-fareast;mso-hansi-font-family:Calibri;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:"Times New Roman";mso-bidi-theme-font:minor-bidi;mso-font-width:90%;mso-fareast-language:ES;font-weight:bold;}.MsoChpDefault{mso-style-type:export-only;mso-default-props:yes;font-family:"Calibri",sans-serif;mso-ascii-font-family:Calibri;mso-ascii-theme-font:minor-latin;mso-fareast-font-family:"MS Mincho";mso-fareast-theme-font:minor-fareast;mso-hansi-font-family:Calibri;mso-hansi-theme-font:minor-latin;mso-bidi-font-family:"Times New Roman";mso-bidi-theme-font:minor-bidi;mso-font-width:90%;mso-fareast-language:ES;font-weight:bold;}size:612.0pt 792.0pt;margin:70.85pt 3.0cm 70.85pt 3.0cm;mso-header-margin:36.0pt;mso-footer-margin:36.0pt;mso-paper-source:0;}div.WordSection1{page:WordSection1;}