Response to extracellular ATP is dependent on the expression of AQP2 in renal cells.

PIZZONI A; DI GIUSTO G; RIVAROLA V; CAPURRO C; LOPEZ-GONZALEZ M; FORD P

Mar del Plata

Congreso; Reunión Anual de la Sociedad Argentina de Fisiología 2018; 2018

Sociedad Argentina de Fisiología

Extracellular ATP is a physiological signal that can activate P2 receptorsand trigger intracellular Ca2+ signals to influence cellularbehavior, including migration. We have previously described inrenal cells that intracellular Ca2+ signals can be modulated by theinteraction between AQP2 and the Ca2+ channel TRPV4. Previousworks from our laboratory have also shown that AQP2 expressionaccelerates cell motility and proliferation. The objective of the presentwork was to study the influence of AQP2-TRPV4 interactionin Ca2+ signals elicited by extracellular ATP and its consequencesin cell migration. We used two rat cortical collecting duct cell linesexpressing AQP2 (AQP2-RCCD1) or not (WT-RCCD1). We studiedCa2+ signals using FURA-2 in response to 20 µM of extracellularATP. We found that Ca2+ signals in WT-RCCD1 cells are greaterthan in AQP2-RCCD1 cells (Area Under the Curve; AUCWT: 401 ± 17vs. AUCAQP2: 272 ± 10; n=318 cells of 7 independent experiments.p