IFIBIO HOUSSAY   25014
INSTITUTO DE FISIOLOGIA Y BIOFISICA BERNARDO HOUSSAY
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Chronic Unpredictable Stress in Drosophila as a Preclinical Model for Psychopharmacology Research
Autor/es:
RAMOS HRYB ANA BELÉN; MARIO RAFAEL PAGANI; LINO-DE-OLIVEIRA, CILENE; MAURO RAMIREZ
Lugar:
Córdoba
Reunión:
Congreso; XXXIII CONGRESO ANUAL SAN 2018; 2018
Institución organizadora:
Sociedad Argentina de investigación Neurociencias
Resumen:
Exposition to chronic and unpredictable stress (CUS) plays a significant role in the psychiatric disorders onset. Frequent symptoms in humans include altered locomotion and anhedonia, which can be partially modeled in rodents as preclinical tests used for drug testing. However, frequent failure of predictive validity and animal welfare issues increase initiatives focused on 3Rs. Drosophila is a powerful organism for modeling human diseases mainly due to their phylogenetic relationship with rodents and their labor/cost effective maintenance advantages. We aimed at using D. melanogaster as an alternative preclinical model for stress and psychopharmacological research. Adult D. melanogaster were exposed to CUS with several stressors (random sequences of 24h isolation, 20min heat shock, 5min electric shock and 6h starvation between animals). Another group was treated with 10mM fluoxetine, 5mM diazepam (DIA) or vehicle during starvation stress. At the end of CUS, behavior in the open field (OF) and 2mM sucrose preference (SP) were analyzed. Compared to control, stressed flies exhibited higher mobility, distance, and velocity as well as less time in OF boundaries. In contrast to control group, stressed flies exhibited less SP. No treatment prevented these behavioral disturbances, however, diazepam increased freezing time. Our CUS model contributes to the construction of a stress­ related model meeting face validity where we reproduced some behavioral phenotypes in Drosophila.