A globotriaosylcramide inhibitor prevented the effect of shiga toxin 2 on cell proliferation and migration in renal tubular epithelial cells.

IBARRA CRISTINA; SANCHEZ DIANA; AMARAL MARÍA MARTA; FISHER SIGEL LILIANA KARINA; SILBERSTEIN CLAUDIA

Mar del Plata

Congreso; LXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica, LXVI Reunión Annual de la Sociedad Argentina de Inmunología, Reunión Anual de la Sociedad Argentina de Fisiología.; 2018

Sociedad Argentina de Investigación Clínica, Sociedad Argentina de Fisiología, Sociedad de Inmunología

In Argentina, post-diarrhea Hemolytic Uremic Syndrome (HUS) due to Shiga toxin-producing Escherichia Coli (STEC) is a common cause of acute renal failure in children. Shiga toxin type 2 (Stx2) binds to the globotriaosylceramide (Gb3) receptor on the surface of renal cells. We have previously shown that the compound C-9 (Genzyme),an inhibitor of glucosylceramide synthase, decreased Gb3 expression in renal epithelial cells. The aim of this work was to study whether the C-9 prevents the cytotoxic actions of Stx2 on cell proliferation and cell migration in Vero cultures. Cell proliferation was measured by bromodeoxyuridine (BrdU) uptake, and cell migration was evaluated as the percentage of wound closure in wound healing assay. Incubation of Vero cultures with Stx2 (10 nM, 24 h) significantly decreased the BrdU uptake to 48 ± 18 % (p