IFIBIO HOUSSAY   25014
INSTITUTO DE FISIOLOGIA Y BIOFISICA BERNARDO HOUSSAY
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Regional microgliosis in transgenic mice expressing a mislocalized form of TDP-43: implications for neurodegenerative disease pathogenesis
Autor/es:
NIEVA GV; IGAZ LM; SILVA PR
Lugar:
Cordoba
Reunión:
Congreso; XXXIII Congreso Anual de la Sociedad Argentina de Investigación en Neurociencias (SAN); 2018
Institución organizadora:
Sociedad Argentina de Investigación en Neurociencias (SAN)
Resumen:
Activated microglia is auniversal feature of frontotemporaldementia (FTD) and amyotrophic lateral sclerosis (ALS), two neurodegenerativedisorders associated to mislocalization and aggregation of TARDNA-binding protein 43 (TDP-43);however, its role in pathogenesis is not well understood.  We generated and characterized transgenic (TG) mice conditionallyoverexpressing eithernuclear (WT) or cytoplasmic (ΔNLS) forms of human TDP-43 in forebrain neurons. Recently, we showed that hTDP-43-WT mice display higher levels of microglialactivation in hippocampal CA1 region and somatosensory cortex (SSC) respect tocontrols. In this study, we aimed to explore microgliosis in hTDP-43-ΔNLS mice. Weanalyzed microglial (Iba1+) staining in TG mice after 1 month of post-weaninginduction in different brain regions. TG mice showed significant increases intotal % Iba1+ area, microglial cell number and Iba+ cells with activatedmorphology (larger somatic area) in SSC and CA1 region compared to controls. Inaddition, there was a significant increase in mean Iba+ soma area in SSC, withborderline significance in CA1 region. Prefrontal cortex displayed nosignificant differences in any of the parameters analyzed. We are currentlyevaluating microgliosis in additional regions, including motor cortex anddentate gyrus, and also the status of astroglial response using GFAP staining.These results will help elucidate the role of gliosis in ALS, FTD and other TDP-43proteinopathies.