IFIBIO HOUSSAY   25014
INSTITUTO DE FISIOLOGIA Y BIOFISICA BERNARDO HOUSSAY
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Effect of nitrative stress on placental AQP9 and its role in the pathogenesis of preeclampsia
Autor/es:
MEDINA YOLLYSETH; REPETTI, JULIETA; DI PAOLA MAURICIO; CASTRO PARODI MAURICIO; ANUD CAROLINA ; DAMIANO ALICIA E
Lugar:
Mar del Plata
Reunión:
Congreso; Reunión Anual Sociedad Argentina de Investigación Clínica; 2018
Institución organizadora:
Sociedad Argentina de Investigación Clínica
Resumen:
Preeclampsia is a gestational hypertensive syndrome of unknown etiology unique to human pregnancy. Increased levels of reactive nitrogen species (RNS) were described in preeclamptic placentas. RNS promotes the formation of peroxynitrite (ONOO-), that covalently modify proteins by nitration of tyrosine residues [3 nitrotyrosine (3NTp)] and lead to a non-functional protein. Nitration of a wide range of placental proteins were found increased in preeclampsia. Previously, in these placentas we reported an overexpression of AQP9 with a lack of functionality.Our aim was to evaluate the effect of nitrative stress mediated by ONOO- on placental AQP9 expression and functionality. This study was approved by the ethics committee of the Hospital Nacional Dr. Prof. A. Posadas. Explants from normal term placentas were cultured under conditions of nitrative stress induced by 100 µM of ONOO-. The cell viability, cytotoxicity, and cell damage indexeswere evaluated. AQP9 expression and water uptake were also studied. The amount of 3NTp-AQP9 in trophoblast from preeclamptic and normal patients was determined using an immunoprecipitation assay. In the presence of 100 µM of ONOO-, AQP9 protein expression significantly increased (n=7; p