IFIBIO HOUSSAY   25014
INSTITUTO DE FISIOLOGIA Y BIOFISICA BERNARDO HOUSSAY
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
HYPEROSMOLAR STRESS MODULATES EXTRAVILLOUS TROPHOBLAST CELLS DIFFERENTIATION
Autor/es:
DAMIANO A.; MARTÍNEZ N.; REPPETTI J.
Lugar:
Bio Bio- Chillán
Reunión:
Congreso; III MEETING ON RESEARCH AND INNOVATION IN VASCULAR HEALTH V RESEARCH MEETING ON HYPERTENSION IN PREGNANCY; 2019
Resumen:
The placenta is a transient organ which serves important functions during the development of the fetus. Trophoblast cells develop along a multilineage differentiation pathway, and their growth and differentiation are essential to the establishment and maintenance of pregnancy.The proliferation, migration, invasion and the remodeling of the spiral arteries by the extravillous trophoblast (EVT) are critical processes for a successful pregnancy. Alterations in any of these events can lead to gestational pathologies such as preeclampsia. Many authors have demonstrated that the hyperosmolarity of the medium induced cells differentiation in different tissues.Our hypothesis is that the stress hyperosmolar modulates EVT during placentation.AimsThe aim of this study was to explore the effect of the stress hyperosmolar on the EVTs migration, invasion and ENDOVASCULAR DIFFERENTIATION.MethodsSwan-71 cell line (human extravillous trophoblast cells) was cultured in complete DMEM-F12 and sucrose hyperosmolar solution was added for 24 h.Cell migration was evaluated by the wound healing assay, activity of metalloproteinases (MMPs) by zymography, the invasion was evaluated in transwells pre-coated with Matrigel® and endovascular differentiation was analyzed by the formation of tube-like structures in plates coated with Matrigel.ResultsIn hyperosmolar conditions, Swan-71 cell migration, MMPs activity, and invasion decreased (33 ± 4%, 75 ± 8%, and 45 ± 6%, respectively compared to iso-osmolar condition; P