Study of cortical oscillatory activity in the development of dyskinesias associated to L-Dopa treatment in a rodent model of Parkinson's disease

MARIANO ANDRÉS BELLUSCIO; DANIELA PIÑA NOVO; MARIO GUSTAVO MURER

Mar del Plata

Congreso; XXXII Congress of the Argentine Society for Research in Neuroscience; 2017

Sociedad Argentina de Investigación en Neurociencias

Prolonged treatment with L-Dopa in Parkinson´s disease (PD) often leads to the emergence of abnormal involuntary movements known as L-Dopa-induced dyskinesias (LIDs). Recent findings in a rodent model of PD have shown that dyskinetic symptoms are linked to the occurrence of a prominent 80Hz oscillatory local field potential (LFP) activity within the primary motor cortex. Additionally, L-Dopa treatment decreases exaggerated beta (13-30) oscillatory activity, which is exacerbated in parkinsonian state and has been associated with rigidez and bradykinesia. Despite of this significant findings, relationship between motor symptoms and oscillatory activity in the development of LIDs, is not fully understood; and how to control the animal´s behavior, especially in the "on" state of LIDs is a major constraint for multielectrode chronic recordings. To solve this and with the purpose to characterize the relationship of beta and high gamma (70-110 Hz) cortical activity with parkinsonian state and the development of LIDs, we design a new setup based on a mouse spherical treadmill where animals could walk and rotate the body freely but had the head restrained of movement. This allowed to recording cortical LFP and spike signals in hemiparkinsonian mice as well in those treated with L-dopa for 7 days, both in the "on" as in the "off" state. Position was also tracked which allowed to correlate the oscillatory activity recorded with behavior. Preliminary results of this will be presented.