IFIBIO HOUSSAY   25014
INSTITUTO DE FISIOLOGIA Y BIOFISICA BERNARDO HOUSSAY
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Role of TRPV4 and AQP2 expression in renal cortical collecting tubule cell growth in acidosis.
Autor/es:
PORTA MICAELA; ALEJANDRO PIZZONI; CLAUDIA CAPURRO; GISELA DI GIUSTO; PAULA FORD; MAZZOCCHI MARINA; NATALIA BELTRAMONE; VALERIA RIVAROLA
Lugar:
Buenos Aires
Reunión:
Congreso; Reunión Conjunta de Sociedades de Biociencia; 2017
Institución organizadora:
SAIC, SAFIS y otras
Resumen:
As mechanisms of proliferation and intracellular pHi are associated is possible that a chronic alteration of the pH modifies cell growth. We previously showed in renal cells that aquaporin 2 water channel (AQP2) expression prevents inhibition of cell growth in acidic media. Ca2+ channel TRPV4 has been proposed as one possible acid sensor. Our previous work showed that TRPV4 and AQP2 interact. So, the aim of this study was to investigate whether renal cell growth in acidic media was dependent on TRPV4. To investigate this we used two renal cortical collecting duct cell lines: WT-RCCD1, not expressing AQPs, and AQP2-RCCD1 expressing constitutive AQP2 in the apical membrane. Cell Growth studies (CG) were performed in control and acid media in presence of vehicle or the TRPV4 inhibitor Rutenium Red (RR). At physiological pH inhibition of TRPV4 decreased both lines cell growth about 20%. .After 24h exposure to an acidic media (ΔpH = 0.4) only WT cells decreased its growth and this response was not modified by RR (%CG Acid = -327 vs Acid + RR= -379, ns n=18). On the other hand, while acid media did not alter AQP2 expressing growth acid + RR significantly decreased cell growth (%CG Acid =-34 vs Acid + RR= -5115, p