Shiga toxin type 2 suppresses human extravillous trophoblast migration and invasion

DAMIANO, ALICIA; GARCIA, CECILIA; IBARRA, CRISTINA; SCALISE, MARÍA LUJAN; SACERDOTI FLAVIA

CABA

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

During human placentation, the extravillous trophoblast (EVT) invadesthe maternal decidua and reconstructs maternal spiral arteries.We have previously reported that Shiga toxin producing Escherichiacoli (STEC) infections during pregnancy may cause maternalor fetal damage mediated by Shiga toxin type 2 (Stx2). We proposethat an early STEC infection during pregnancy could damage humantrophoblast mediated by Stx2. The aim of this work was to evaluatethe effects of Stx2 on cell viability, migration and invasion ofhuman EVT. Swan 71 cell line was used as human first trimestertrophoblast model. Firstly, cells were submitted to a lipid extractionfor globotriaosylceramide (Gb3, Stx2 receptor) analysis by thin layerchromatography. Cell viability was evaluated by neutral red uptakeat 24, 48 and 72h of treatment with Stx2 (0.001-1μg/ml). To assessthe rates of apoptosis, cells were fluorescently stained with orangeacridine/ethidium-bromide and analyzed under optical microscopy.The effects of Stx2 on migration were studied by the wound-healingassay at 8 and 24h. Proteine activity of matrix metaloproteinase-2(MMP-2) and invasiveness were analyzed at 24h after treatmentby zymography and Matrigel invasion assay, respectively. Gb3 wasexpressed in Swan 71. Stx2 (1μg/ml) inhibited cell viability andincreased the apoptotic rate at 72h respectively (p