Nrf2-ARE SIGNALING PATHWAY ASSOCIATED TO ANTIOXIDANT RESPONSE IN RAT BRAIN AFTER ACUTE IRON AND COPPER OVERLOADS

REPETTO, MARISA; SAPORITO-MAGRIÑÁ, CHRISTIÁN; FUDA, JULIÁN; BOVERIS, ALBERTO; MUSACCO-SEBIO, ROSARIO; CASTRO-PARODI MAURICIO; FERRAROTTI, NIDIA; ACOSTA, JUAN MANUEL; DAMIANO, ALICIA E; TORTI, HORACIO

Paris

Conferencia; 19th International conference on Oxidative stress reduction, redox homeostasis and antioxidants; 2017

International Society of Antioxidants

Introduction: Thetranscription factor Nrf2-ARE signaling pathway is involved in detoxification and eliminationof reactive oxidants through antioxidant and conjugation reactions and byenhancing cellular antioxidant content1. The molecule that seems tobe the signal for the genomic transcription is a soluble phospholipidhydroperoxide (ROOH). Materials & Methods: Rats(220 ± 8 g) were given single doses of 30 mg/kg Fe or 5 mg/kg Cu. Theactivities and expressions of brain cytosolic superoxide dismutase (SOD1),catalase and levels of Nrf2 in brain and the ratio of reduced/oxidizedglutathione (GSH/GSSG) was determined in the period of 0-24 h after metals ionsloads.Results: SOD1, catalase,glutathione transferase and Nrf2 increased their expressions in response tooxidative stress: SOD1, 2.5 and 1.8 times; catalase 2 and 2.5 times after 24 h (p