Microarray analysis of gene expression in human Muller cells in response to hypotonicity

FORD PAULA; RAMOS MANDUJANO GERARDO ; NETTI VANINA; CAPURRO CLAUDIA

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

Müller cells are involved in controlling extracellular homeostasis in the retina, where neural activity alters osmotic gradients, leading to cell swelling and consequently to a regulatory volume decrease (RVD) response. We previously demonstrated in human Müller cells (MIO-M1) that RVD depends on the efflux of KCl, Taurine and Glutamate, followed by water outflow through Aquaporin-4 (AQP4). Hypotonic shock (HS) also induces a slight transient increase in intracellular Ca2+ levels and membrane depolarization and repolarization. However, the mechanisms for long-term adaptation to hypotonicity are largely unknown. Thus, the aim of this study was to identify HS-related changes in gene expression triggered by cell swelling. Gene transcription by MIO-M1 cells exposed to control or HS (ΔOsm:100 mOsm) for 30 minutes was compared using Affymetrix microarray for 10000 genes. Microarray data analysis was performed with GenArise software and DAVID Bioinformatics Resources 6.8 Database. HS induced the upregulation (Z-score>2SD) of 241 genes and downregulation (Z-score