IFIBIO HOUSSAY   25014
INSTITUTO DE FISIOLOGIA Y BIOFISICA BERNARDO HOUSSAY
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Hyperosmotic stress modulates the expression and function of AQPs in human amnion
Autor/es:
IBARRA C; SIERRA M, ANUD C, DOROSSHNKO B; DI PAOLA M; DAMIANO A
Lugar:
Buenos Aires
Reunión:
Congreso; Reunión Conjunta de Sociedades de Biociencia; 2017
Institución organizadora:
Sociedad Argentina de Investigación Clínica
Resumen:
Amniotic fluid is essential for normal fetal growth and development. The expression of aquaporins (AQP) 1, 3, 8 and 9 has been described in fetal membranes and it was proposed that they could play a key role in amniotic fluid homeostasis. Emerging data correlates the abnormal expression of amnion AQPs with pathological alterations of amniotic fluid volume such as the oligohydramnios or polyhydramnios. Recently, we demonstrated that AQPs facilitate the water transport across the human amnion, being the AQP1 the most important contributor. However, the regulation of the amnion AQPs by changes in the osmolarity was not studied yet. The aim of this work was to studythe effect of hyperosmolar stress on the expression and function of the AQPs present in the human amnion. This study was approved by the ethics committee of the Hospital Nacional Dr. Prof. Alejandro Posadas. Human amnion explants were cultured in complete DMEM-F12 and different sucrose hyperosmolar solutions were added to generate the hyperosmolar conditions. The expression of AQP 1, 3 and 9 was analyzed by the Western Blot and PCR. Amnion explants were mounted in a modified Ussing chamber, net transepithelial water movement was recorded with andwithout an osmotic gradient, and osmotic permeability (pOsm) was calculated. In amnion explants cultured in 400 mOsm (hyperosmolar condition), we observed that AQP1 expression decreased 80.0% ± 4,8%, while AQP3 diminished 42.2% ± 11.6% and AQP9 72.4% ± 1.3% compared to those explants cultured in isoosmolar condition (300 mOsm) (n=5, p< 0.05). In agreement with these results, POsm also decreased 50.0% ± 4.7% (n=5, p