IFIBIO HOUSSAY   25014
INSTITUTO DE FISIOLOGIA Y BIOFISICA BERNARDO HOUSSAY
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Aquaporin 1 and 4 expressions before and after cardiopulmonary bypass during aortic valve replacement surgery in adults.
Autor/es:
FERREYRA R; C . CAPURRO; V NETTI; PIAZZA A,; M T POLITI; BORTMNAN G
Lugar:
Buenos Aires
Reunión:
Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017
Resumen:
Cardiopulmonary bypass (CPB) is an essential strategy in heart valve replacement surgery. However it may produce postoperative myocardial edema, which has been associated to myocardial dysfunction and worst clinical outcomes. Aquaporin (AQP) 1 and 4 are selective water channels expressed in myocardial tissue. In animal models AQP1 increases its expression after CPB, playing an important role in the formation of myocardial edema; information in humans is still scarce. We designed an observational study to evaluate changes in AQP1 and 4 expression before and after CPB in adults undergoing aortic valve replacement surgery. Objectives: 1) To quantify changes in AQP 1 and 4 expression in myocardial tissue before and after CPB using Western blot technique. 2) To correlate changes in AQP expression with clinical characteristics and outcomes. Methods: Patients with aortic valve stenosis undergoing valve replacement surgery were invited to participate. Patients with previous myocardial infarction, primary cardiomyopathy, other concomitant severe valve diseases or combined surgery were excluded. Biopsies were taken from the anterior wall of the left ventricle before and after CPB using an automatic transmural fine-needle. Western blot analysis was performed using anti-AQP1 and anti-AQP4 antibodies. Patients were followed until discharge. The protocol was approved by an IRB. Results: 14 patients were enrolled between August 2015 and June 2017. Patients were mostly elder (70±9.13 years-old) males (62.5%) with hypertension (75%) and degenerative aortic stenosis (86%). Mean biopsy weight was 14±6.9 mg. Western blot showed a mean increase in the 45 KDa (0.57±1.11), 35 KDa (0.30±0.64) and 28 KDa bands (0.33±0.79) of AQP1, and a decrease in the expression of AQP4 (0.61±0.77). Changes in AQP expression were associated with moderate-severe hypertrophy and on-pump time. Conclusion: The expression of AQP1 increases while the expression of AQP4 decreases before and after CPB.