HYPEROSMOLAR STRESS AFFECTS TRPV-1 EXPRESSION AND THE PHYSIOLOGICAL FUNCTIONS OF HUMAN TROPHOBLAST

N. SZPILBARG; N. MARTÍNEZ,; J. REPPETTI ; M. FARINA; A. SEHAYIAN; A.E. DAMIANO

Puerto Varas

Simposio; Latin American Society for Maternal Fetal Interaction and Placenta; 2017

SLIMP

Hyperosmolar stress may be an important stressor that alters normal development of embryos or placentation. TRPV-1 is activated by hyperosmolarity and participates in many cellular processes like apoptosis, authophagy and so others.OBJECTIVE: To evaluate the effect of hyperosmolar stress on cell viability, migration and invasion and the contribution of TRPV-1 to these processes in first trimester human trophoblast cells.METHODS: Swan-71 cell line (human trophoblastic cells) was cultured in complete DMEM-F12 and sucrose hyperosmolar solution was added for 24h with or without capsaicin (CPZ) and capsazepine (CPZ). TRPV-1 protein expression was analyzed by Western blot. Cell viability was analyzed by MTT, LDH and β-hCG assays. Apoptosis was evaluated by Bax expression, DNA fragmentation and TUNEL assay and authophagy by monodansylcadaverine (MDC) assay. Migration was assessed by wound healing assay, activity of metalloproteinases (MMPs) by zymography and invasion was evaluated in transwells pre-coated with Matrigel®. RESULTS: In hyperosmolar conditions, TRPV-1 expression was increased (p