Influence of communication between human renal endothelial and epithelial cells on pro-inflammatory mediators induced by Escherichia coli Shiga type 2 and Subtilase toxins.

TOWSTYKA NADIA YASMÍN TOWSTYKA; AMARAL MARÍA M; SHIROMIZU CAROLINA MAIUMI ; IBARRA CRISTINA; ALVAREZ ROMINA S; JANCIC CAROLINA

Ciudad Autónoma de Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

SAIC, SAIB, SAI, SAA, SAB, SAB, SAFE, SAFIS, SAH, SAP.

Hemolytic Uremic Syndrome associated to Shiga toxin (Stx)-producing E. coli infection is the most common cause of acute renal failure (ARF) in children in Argentina. Stx type 2 (Stx2) damages human renal microvascular endothelial cells (HGEC) and tubular epithelial cells (HK-2) and induces a broad endothelial inflammatory response that injures the tubules. Subtilase cytotoxin (SubAB) may also contribute to this pathology. We developed HGEC/HK-2 coculture as a model of renal proximal tubule to clarify the involvement of the endothelial-epithelial cross talk in kidney toxins damages. In this work, we evaluated the response of selected pro-inflammatory mediators when HGEC, HK-2 and HGEC/HK-2 were incubated with Stx2 (0.01ng/ml), SubAB (1ng/ml) or Stx2+SubAB [(0.01+1) ng/ml] for 24 h. Culture supernatants were collected and IL-6, IL-8 and TNF-α were quantified by ELISA (Stx2, SubAB, Stx2+SubAB vs Ctrol, n=4). Controls showed a differential release of IL-6, IL-8 and TNF-α between monocultures and cocultures, suggesting that soluble mediator?s secretion depends on the cell type and culture conditions (p