IFIBIO HOUSSAY   25014
INSTITUTO DE FISIOLOGIA Y BIOFISICA BERNARDO HOUSSAY
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
CEREBELLAR MICROVASCULAR AND GLIAL ALTERATIONS DURING THE ENCEPHALOPATHY PRODUCED BY SHIGA TOXIN 2 (Stx2) FROM ENTEROHEMORRHAGIC Escherichia coli (EHEC)
Autor/es:
JAN MARCOS KALINSKI; JORGE GOLDSTEIN; VANINA GISELLE VELARDO; CLARA VALENTINA BERDASCO
Reunión:
Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017
Resumen:
Hemolytic uremic syndrome is a triad of events that includes thrombocytopenia, microangiopathic hemolytic anemia and acute renal failure caused by Stx2 from enterohemorrhagic Escherichia coli (EHEC). It has been observed that Stx2 produces deficits of coordination and equilibrium in patients which suggest cerebellar impairment. The aims of this study were to determine whether Stx2:i. passed through the blood-cerebrospinal fluid barrier into cerebellarparenchyma and ii. caused cell damage. Male NIH mice (n=4) were injected intravenously with 1ηg of Stx2 per mice or 100 µl of saline solution (control group). After 4 days of treatment, fixed brains were subjected to immunofluorescence with lectins to determine the microvasculature profile, anti-Stx2 to identify the presence of the toxin in cerebellum tissue, and anti-GFAP (Glial Fibrillary Acidic Protein) to determine reactive astrocytes. Micrographs were taken in a confocal microscope and obtained images were analyzed through the software ImageJ. Stx2 was found in parenchyma cells of the cerebellum. The statistical results analyzed by Student?s t-test showed that Stx2 decreased the area occupied by the microvasculature (18.78 ±0.67 µm2 control versus 9.37 ±0.42 µm2 Stx2) and increased the expression levels of GFAP in comparison to the control (20.32±0.41 AU control versus 29.05 ±0.48 AU Stx2, in IOD) p