AQP3 IS REQUIRED FOR EXTRAVILLOUS TROPHOBLAST MIGRATION DURING PLACENTATION

ABRIL SEYAHIAN; NORA MARTÍNEZ; NATALIA SZPILBARG; ALEJANDRA RECA; ALICIA E DAMIANO; JULIETA REPPETTI

Ciudad Autónoma de Buenos Aires

Congreso; Reunión conjunta de Sociedades de Biociencias; 2017

AQP3 IS REQUIRED FOR EXTRAVILLOUS TROPHOBLAST MIGRATION DURING PLACENTATIONDevelopment of the human placenta is critical for a successful pregnancy. It requires that the extravillous cytotrophoblast cells (EVTs) migrate, invade and transform maternal spiral arteries. It was recently reported that aquaporin 3 (AQP3) is expressed in trophoblast cells during early gestation and its expression decreased dramatically in preeclamptic placentas. Although, AQP3 is involved in the migration of different cell types, its role in human placenta is still unknown.Objective: To evaluate the role of AQP3 in the migration and the invasion of EVT.Methods: Trophoblast cells (Swan 71 cell line) were cultured in complete DMEM-F12. AQP3 expression was examined by Western blot, RT-PCR and immunofluorescence. Cells were also treated with 100 µM CuSO4 (AQP3 specific blocker) or SiRNA-AQP3 to inhibit AQP3. Migration was assessed by wound healing assay. Transwell invasion assay was also performed. Metalloproteinase activities were evaluated by gelatin gel zymography. Results: AQP3 expression was found in Swan-71. Wound healing assay: the closure of the gap expressed as Control vs CuSO4 (n=5) at each time point was: 8 h (20.77 ±1.23 vs 14.44± 1.21, p