Effects of estradiol on cell proliferation and cyclins D1 and B1 expression in primary cultures of human renal epithelial cells

CLAUDIA SILBERSTEIN; KEVIN D`ELÍA; ELISABET M ODDO; DAIANA S SANCHEZ; FERNANDO R IBARRA; PABLO J AZURMENDI

CABA

Congreso; Reunión Conjunta de Sociedades de Biociencia; 2017

The proximal renal tubule epithelium expresses receptors for estradiol, however, little is known about its functions in the kidney. A sexual dimorphism was found in compensatory renal growth, due to the female sex hormones, but the hormonal role in this process is not clarified. The mechanism of cell proliferation is part of the epithelial regeneration process in renal tubules. The objective of the work was to study the effects of estradiol on cell proliferation and the expression of intracellular signals involved in cell cycle, in primary and three-dimensional (3D) cultures of human cortical renal tubular epithelial cells (HRTEC), developed from nephrectomies performed in pediatric patients at the Hospital Nacional Prof. A. Posadas. HRTEC proliferation rate was evaluated by incorporation of bromodeoxyuridine (BrdU), mRNA expression for cyclin D1 (CYD1) and B1 (CYB1) was assessed by RT-qPCR and tubulogenesis was performed in 3D-HRTEC cultures. HRTEC Incubation with 17β-estradiol (17BE 10 nM, 24 h) stimulated the incorporation of BrdU by 40 ± 4,7 % (p