IFIBIO HOUSSAY   25014
INSTITUTO DE FISIOLOGIA Y BIOFISICA BERNARDO HOUSSAY
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Expression of proteins related to sodium balance in renal medulla and in peripheral blood lymphocytes in a rat model of salt-sensitive hypertension
Autor/es:
LUIS A DI CIANO, ELIZABET M ODDO, ELVIRA E ARRIZURIETA, NORA P GOETTE, PABLO J AZURMENDI; CLAUDIA SILBERSTEIN; FERNANDO R IBARRA; SANDRA G VLACHOVSKY
Lugar:
CABA
Reunión:
Congreso; Reunión Conjunta de Sociedades de Biociencia.; 2017
Resumen:
The absence of female sex hormones in ovariectomized (oVx) rats alters renal medulla expression of Na+, K+- ATPase (NKA), Na+ K+ 2Cl- cotransporter (NKCC2) and D1 dopamine receptor (D1DR). During high salt intake sodium excretion is deficient and rats develop salt sensitive hypertension (SSH). The immune system plays a role in arterial hypertension. In lymphocytes, NKA and NKCC2 are associated with cell growth / proliferation. Therefore, the aim of the present work was to study the expression of NKA, NKCC2 and D1DR proteins in the absence of female sex hormones in peripheral blood mononuclear cells (90% of lymphocytes, PBL) from intact female (IF) and ovariectomized (oVx) Wistar rats. The oVx was performed at 60 days of life and rats were studied 90 days post-oVx. During the last 5 days rats received normal (NS, NaCl 0.24%) or high salt intake (HS, NaCl 1%). At day 150, mean arterial pressure (MAP) was recorded and peripheral blood mononuclear cells were isolated by Ficoll gradient. The expression of D1DR, NKCC2, total NKA and NKA dephosphorylation state (NKA is more active when dephosphorylated at Ser 23) was analyzed by Western blot.Total NKA decreased in IF with HS intake (p