Thalamic neurological alterations caused by STx2 and LPS in a murine model of hemolytic uremic syndrome encephalopathy

PINTO ALIPIO; CANGELOSI ADRIANA; ARENAS DAVID.; GEOGHEGAN PATRICIA; BERDASCO CLARA; GOLDSTEIN JORGE

Simposio; The role of glia in health and disease of the nervous system: Clinical and basic science walking together; 2017

Hemolytic uremic syndrome is produced by enterohemorrhagic E. coli that secretes the neurotoxins Shiga toxin (Stx) and lipopolysaccharide (LPS). In order to determine the alteration of the neurovascular unit of the anterior and lateral ventral nuclei of the thalamus by these toxi ns, Swiss-NIH male mice were used and Stx2 and / or LPS or vehicle were inoculated and, on the fourth day of the treatment, intracardiac perfusion. immunofluorescence was performed: anti-NeuN, anti-GFAP to assess astrocytic reaction, Lectins, GB3 and Hoechst. Images were captured with fluorescence microscopy, analysis with the ImageJ program, and statistic with Test Anova and Bonferroni's Multiple Comparison. The number of neuronal nuclei with abnormal phenotype was significant (p