IFIBIO HOUSSAY   25014
INSTITUTO DE FISIOLOGIA Y BIOFISICA BERNARDO HOUSSAY
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Purinergic receptors and neuronal progenitor cell regulation in the adult retina of zebrafish
Autor/es:
FAILLACE MARÍA PAULA
Lugar:
Valparaíso
Reunión:
Simposio; Third Meeting of the Zebrafish Latinoamerican Network; 2014
Institución organizadora:
Zebrafish Latinoamerican Network (LAZEN)
Resumen:
Purinergic receptors and neuronal progenitor cell regulation in the adult retina of zebrafish Maria Paula Faillace Instituto de Fisiología y Biofísica Bernardo Houssay (IFIBIO-Houssay) UBA, CONICET. Departamento de Fisiología, Facultad de Medicina, Universidad de Buenos Aires,  Argentina. Instituto de Química y Fisicoquímica Biológicas (IQUIFIB) - CONICET.   Cell extrinsic purinergic signals are essential for regulating cell proliferation, migration, differentiation and death in the development of vertebrate nervous system. On the other hand, fish can regenerate diverse tissues including the retina. The capacity of the adult zebrafish retina to grow and regenerate, unlike mammal´s retina, has served as a model to study cellular and molecular mechanisms that sustain retinal plasticity. We aimed to investigate the purinergic system role in retinal repair following cytotoxic injuries that provoke severe cell death. To this end, we examined whether retinal progenitor cells were able to proliferate and differentiate by inhibiting nucleotide binding to their specific receptors. Our results indicated that an ecto-ATPase activity is essential for amplifying neuronal progenitor cells necessary for retinal repair. In addition, ADP-activated purinergic receptors P2Y1 (P2Y1R) are not only involved in the neuronal progenitor amplification process, which provides a sufficient number of precursor cells, but likely in the activation of multipotent Müller cells, from which neuronal progenitors originate following damage. After injury, P2Y1R, showed enhanced levels and a modified distribution in the retinal layers. Treatment with an antagonist of P2Y1R provoked a drastic decrease in this receptor protein levels. ADPßS was able to increase P2Y1R mRNA, providing a possible mechanism throughout which this purinergic receptor expression was induced by the injury. At present, we investigate whether the purinergic system is involved in mutipotent Müller cell activation and cell fate determination by studying homeobox and transcription factor (bHLH) expression. Grants: PIP 0169 2009-2011 and PIP 079CO 2012-2014 CONICET - UBACYT 0823 2011-1014, Universidad de Buenos Aires, Argentina.