IFIBIO HOUSSAY   25014
INSTITUTO DE FISIOLOGIA Y BIOFISICA BERNARDO HOUSSAY
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Extracellular nucleotide receptors and ecto-ATPase activity are involved in the induction and amplification of neuronal progenitor cell number for retinal regeneration in zebrafish.
Autor/es:
MATÍAS P. MEDRANO, ARIADNA G. BATTISTA, CLAUDIO A. BEJARANO, GRACIELA D. VENERA, RAMÓN O. BERNABEU AND MARIA PAULA FAILLACE
Lugar:
VALPARAISO
Reunión:
Workshop; Latin American Zebrafish Network 3rd meeting; 2014
Resumen:
Extracellular
nucleotide receptors and ecto-ATPase activity are involved in the induction and
amplification of neuronal progenitor cell number for retinal regeneration in
zebrafish.
Authors: Matías P. Medrano1,2,◊, Ariadna G. Battista1,2,◊,
Claudio A. Bejarano1,2, Graciela D. Venera2, Ramón O.
Bernabeu1,3 and Maria Paula Faillace1,2,*.
Institutionalaffiliations:
1.
Departamento de Fisiología, Facultad de Medicina, Universidad de Buenos Aires
(UBA), Buenos Aires, Argentina.
2.
Instituto de Química y Fisicoquímica Biológicas (IQUIFIB), Consejo Nacional de
Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
3.
Instituto de Biología Celular y Neurociencias Prof. Eduardo De Robertis. UBA-CONICET, Buenos Aires, Argentina.
Here we described that the activity of ectonucleoside
tri-phosphate diphosphohydrolases (NTPDases), which hydrolyze extracellular
nucleotides, regulate daughter progenitor cell proliferation following Müller
glia activation for regeneration of the adult zebrafish retina. In addition,
ADP-activated P2Y1 receptors(P2Y1R) were key regulators
of cell cycle at the interval in which Müller cell activate and daughter
progenitor cell population amplify for retinal repair. We detected apoptotic
cells on days 3-7 post injury. However, either antagonism of the P2Y1R
or inhibition of ecto-ATPase, including NTPDase, activity did not affect the
number of apoptotic cells. Furthermore, we first described several P2 receptors
expressed by the adult zebrafish retina at the transcriptional level.
Regenerating retinas showed a significant increase of P2Y1R mRNA
expression in the peak of the proliferative activity. Additionally, P2Y1R
mRNA levels in the intact retina were enhanced by in vivo exposure to ADPbS, whereas ATPgS was ineffective. This suggested that P2Y1R expression
following injury could result from endogenous ADP extracellular signaling. In
the zebrafish retina and brain, P2Y1R protein was detected by
western blot as a 63 kDa monomer like the form exhibited by rat and human brains.
After injury, P2Y1R, assessed by immunocytochemistry, showed
enhanced levels and a modified distribution in the retinal layers. Moreover,
treatment following damage with an antagonist of P2Y1R provoked a
drastic decrease in this receptor protein levels. Some BrdU-positive retinal
cells might express P2Y1R suggesting a direct effect of
extracellular nucleotides on their mitotic activity. Furthermore, NTPDase1, 2
and 3 mRNA exhibited augmented levels following injury.
Grant sponsors: Agencia Nacional de
Promoción Científica y Tecnológica (ANPCyT)-FONCyT Préstamo BID1728/04-PICT
2004 No25422. Consejo Nacional de Investigaciones Científicas y Técnicas
(CONICET) PIP 2009-2011 N° -0169. UBACYT
2011-2014 N° -0823, University of Buenos Aires. ARGENTINA.