IFIBIO HOUSSAY   25014
INSTITUTO DE FISIOLOGIA Y BIOFISICA BERNARDO HOUSSAY
Unidad Ejecutora - UE
artículos
Título:
In Vivo Photodynamic Therapy With a Lipophilic Zinc(II) Phthalocyanine Inhibits Colorectal Cancer and Induces a Th1/CD8 Antitumor Immune Response
Autor/es:
DUHALDE VEGA, MAITE; DAGHERO, HELLEN; GARCÍA VIOR, MARÍA C.; CHIARANTE, NICOLÁS; ZOTTA, ELSA; BOLLATI?FOGOLIN, MARIELA; ROGUIN, LEONOR P.; VALLI, FEDERICO; BASIKA, TATIANA; MARINO, JULIETA; DUHALDE VEGA, MAITE; DAGHERO, HELLEN; GARCÍA VIOR, MARÍA C.; CHIARANTE, NICOLÁS; ZOTTA, ELSA; BOLLATI?FOGOLIN, MARIELA; ROGUIN, LEONOR P.; VALLI, FEDERICO; BASIKA, TATIANA; MARINO, JULIETA
Revista:
LASERS IN SURGERY AND MEDICINE
Editorial:
WILEY-LISS, DIV JOHN WILEY & SONS INC
Referencias:
Lugar: New York; Año: 2020 vol. 53 p. 344 - 358
ISSN:
0196-8092
Resumen:
Background and Objectives: Photodynamic therapy(PDT) is an antitumor procedure clinically approved forthe treatment of different cancer types. Despite strongefforts and promising results in this field, PDT has not yetbeen approved by any regulatory authority for the treatmentof colorectal cancer, one of the most prevalent gastrointestinaltumors. In the search of novel therapeuticstrategies, we examined the in vivo effect of PDT with alipophilic phthalocyanine (Pc9) encapsulated into polymericpoloxamine micelles (T1107) in a murine coloncarcinoma model.Study Design/Materials and Methods: In vivo assayswere performed with BALB/c mice challenged with CT26cells. Pc9 tumor uptake was evaluated with an in vivoimaging system. Immunofluorescence, western blot, andflow cytometry assays were carried out to characterize theactivation of apoptosis and an antitumor immune response.Results: Pc9‐T1107 effectively delayed tumor growth andprolonged mice survival, without generating systemic ortissue‐specific toxicity. The induction of an apoptotic responsewas characterized by a decrease in the expressionlevels of Bcl‐XL, Bcl‐2, procaspase 3, full length Bid, a significantincrement in the amount of active caspase‐3 and thedetection of PARP‐1 cleavage. Infiltration of CD8+ CD107a+T cells and higher levels of interferon‐γ and tumor necrosisfactor‐α were also found in PDT‐treated tumors.Conclusions: Pc9‐T1107 PDT treatment reduced tumorgrowth, inducing an apoptotic cell death and activating animmune response.