INBIOSUR   25013
INSTITUTO DE CIENCIAS BIOLOGICAS Y BIOMEDICAS DEL SUR
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
PTHrP DIFFERENTIALLY REGULATES MIR-423 AND MIR-27a IN NORMAL INTESTINAL CELLS AND COLORECTAL CANCER CELLS
Autor/es:
NOVOA DIAZ MARÍA BELÉN; CONTRERAS HECTOR; CARRIERE PEDRO; MORALES SEBASTIÁN; GENTILI CLAUDIA; CALVO NATALIA; MARTIN MARIA JULIA
Lugar:
Reunión virtual
Reunión:
Congreso; Reunión Anual de Sociedades de Biociencias SAIC.SAI.SAFIS 2020; 2020
Institución organizadora:
Sociedad Argentina de Investigación Clínica (SAIC)
Resumen:
MicroRNAs (miRs) are small non-coding RNAs that participate in the regulation of mRNAs at the post-transcriptional level in physiological and pathological processes such as cell differentiation, migration, and invasion. The differential presence of the 3p and 5p isoforms originating from the pre-microRNA precursor has recently been correlated with cancer initiation and progression. Previously, we obtained evidence that parathyroid hormone-related peptide (PTHrP), which is overexpressed in colorectal cancer (CRC), induces molecular changes promoting angiogenesis, Epithelial-Mesenchymal Transition (EMT), and cancer stem cell features in CRC cells. The objective of this work was to explore the potential role of PTHrP in the post-transcriptional regulation of mRNAs, evaluating the expression of miR-423 and miR-27a isoforms in the normal intestinal CCD841 CoN cell line and in HCT116 cells derived from colorectal cancer. miR-423 is increased in the plasma of CRC patients, while miR-27a was identified as a tumor suppressor. Employing TaqMan MicroRNA assays by qPCR, we observed that PTHrP treatment for 5 hours significantly increases the miR-423-3p and miR-423-5p transcripts levels in HCT116 cells; however, no changes were evidenced in CCD841 CoN cells at the studied times. In HCT116 cells, the transcription of miR-27a-5p increased at 5 hours of PTHrP exposure with a return to control levels within 24 hours. Differently, PTHrP induced a consistent decrease in miR-27a-3p transcription in HCT116 cells and CCD841 CoN cells at the same times. These results highlight the relevance of the temporal coexistence of the two functional isoforms of these miRs and propose that miR-27a-3p isoform and miR-423 isoforms could be possible post-transcriptional regulators modulated by PTHrP in intestinal tumor cells.