Biofilm Formation on Ultrafiltration Membranes by Yeast Strains Isolated from Apple Juice Processing Industries

AGUSTIN, MARÍA DEL ROSARIO; TARIFA, MARÍA CLARA; BRUGNONI, LORENA INÉS

Bahía Blanca

Conferencia; International Conference of Production Research-Americas; 2020

Departamento de Ingeniería, Universidad Nacional del Sur

Abstract.Four yeast strains (Candida tropicalis, C. krusei, C. kefyr and Rodhotorula mucilaginosa) isolated from an apple juice plant were evaluated for their ability to form monospecies biofilms on ultrafiltration membranes.Yeast attachment and biofilm formation was estimated at different contact times 2, 6, 24, 48, 72 h and one week usingapple juice as food matrix and polyvinyli-dene fluoride membrane as contact surface. The highest counts recorded after one week trial were for C. tropicalis and C. krusei (9.02 ± 0.30 and 9.30 ± 0.29 log CFU cm-2 respectively), followed byC. kefyr (8.63 log CFU cm-2) and R. mucilaginosa with the lowest adhesion (7.68 log CFU cm-2). The present study supports the importance to examine the interactions among members of resident microbiota on surfaces commonly used forjuice clarification processes andshould alert about improving and adopting better cleaning and disinfection pro-tocols as they can represent a serious hygienic-sanitary problem