Role of the phytoestrogen Genistein on cellular events involved in bone remodeling: bone-vascular cells crosstalk

SANDOVAL MJ; MASSHEIMER VL; CAMPELO AE; CEPEDA SB

Congreso; American Society of Bone and Mineral Research (ASBMR) 2020 Annual Meeting. Virtual Event; 2020

Phytoestrogens (PE) as Genistein (Gen) are proposed as natural therapy for bone loss prevention. We studied the mechanism of action of Gen on osteoblast (OB) differentiation; and on bone-vascular cells interactions with focus on angiogenesis. Aortic rings (AR, ex vivo assays) or murine primary cell cultures (in vitro assays) of aortic endothelial cells (EC) or calvarial OB exposed to 10 nM Gen were employed as experimental models. Using RT-PCR technique, we found that Gen increased estrogen receptor alpha (ERα) and Runx2 mRNA levels at 3-5 days of culture (2; 20 fold a/c, respectively, p