Opposite actions of progesterone (Pg) and medroxyprogesterone acetate (MPA) in bone and vascular cells

MASSHEIMER, VIRGINIA; CUTINI PABLO

MAR DEL PLATA

Congreso; LXIV Reunión Anual de la SAIC; 2019

SAIC

Due to the fact that the incidence of osteoporosis and cardiovascular diseases increases in postmenopausal women, hormone replacement therapy including natural or synthetic progestins such as MPA emerged as a therapeutic option. Vascular calcification developed in atherosclerotic plaque is partly due to the osteogenic transdifferentiation of vascular smooth muscle cells (VSMC). Using murine primary cultures of calvarial osteoblasts (OB) and aortic VSMC, we investigated the effect of Pg and MPA on osteoblastic differentiation markers. In order to induce osteoblastic differentiation, VSMC were cultured for 21 days in osteogenic medium (4 mM CaCl2 and 10 mM β-glycerophosphate). When VSMC were cultured in osteogenic medium (VSMC-OB), treatment with Pg (100 nM) for 21 days induced a significant reduction in ALP activity (25% below control, p