ESTROGEN RECEPTOR ESR1 POLYMORPHISMS AS EARLY PREDICTORS OF PREECLAMPSIA -ECLAMPSIA IN HIGH-RISK PREGNANCIES

PLUNKETT R; MARIA IVONE VALLE; ESPECHE W; RAUSCHEMBERGER M. BELÉN; SALAZAR M; MASSHEIMER VIRGINIA

Buenos Aires

Congreso; International Federation of Placenta Associations 2019 (IFPA2019); 2019

IFPA

Objectives: Hypertensive disorders affect 30% of high-risk pregnancies. The study of polymorphisms of estrogen receptors as early markers of preeclampsia (PE) has shown discordant results. The aim of the present work was to investigate the existence of associations between polymorphisms of the estrogen receptor ESR1 (PvuII and XbaI) with Pre-clampsia, Helpp, Eclampsia, in a high-risk pregnant women population from San Martín Hospital of La Plata (Buenos Aires, Argentina). Methods: Pregnant women (n=81) with high risk factors for Preeclampsia were evaluated prior to 20 weeks of gestation. ESR 1 polymorphic variants were studied by RFLP-PCR technique. Amplified products of target region (intron 1) were subjected to cuts PvuII and XbaI enzymes and resolved by electrophoresis in agarose gels.Results: The clinical profile of the 81 patients: average age 29.6 years, 2 patients with previous diabetes, 8 patients with previous hypertension; at week 30: average BP by 24-hour presurometry: 127/77, nocturnal BP found greater than 120/70: 31 cases, 12 cases of preeclampsia, 64 live births. The genotypes determined for PvuII classified as: 1 (C/T); 2 (T/T) and 3 (C/C), showed frequencies of 29; 59 and 12% respectively, while in the case of XbaI, genotype A (A/G) is present in 35.3% of the analysed population, genotype B (A/A) in 2%, and genotype C ( G/G) in 62.7%. The major haplotype present in the population studied was 2C. In contrast in young women without PE risk the key haplotype determined was 1A (Rauschemberger et al, 2012). When plasmatic levels of nitric oxide were measured, we found a significant reduction in the vasoactive concentration in pregnant women that developed eclampsia respect to the other pregnant women studied (16±2,20 vs 34±1,41 vs nmol NO/100mL plasma respectively).Conclusion: The results suggests that pregnant women with high PE risk exhibit different polymorphic variants of ESR1 respect to non- risk pregnant women, and a markedly reduction in the main vasoactive responsible of vascular homeostasis maintenance.