Similar effects of estrone on vascular and bone tissues: ¿Risk or benefit?

RAUSCHEMBERGER, MARÍA BELÉN; CRESCITELLI, MARÍA CARLA; MASSHEIMER, VIRGINIA L.

Mar del Plata, Pcia. de Buenos AIres

Congreso; XLIV Reunión Anual SAIC; 2019

SAIC

It is known that the decrease in estrogen levels during menopause negatively affects bone tissue and vascular function. We have previously shown that E1 modulates the cellular events involved in atherogenesis such as vascular smooth muscle cells (VSMC) proliferation and migration, and monocyte/platelet adhesion to endothelium. Vascular calcification represents the advanced stage of atherogenesis, where VSMC transdifferentiation on bone like cells plays a crucial role. The aim of this work was to compare the effect of 10 nM E1 (48 h) on murine calvarial osteoblasts (OB), and on vascular smooth muscular cells transdifferentiated into osteoblasts (VSMC-OB) by incubation in a procalcification medium (10 mM glycerophosphate) for 21 days. We focused our attention on cell differentiation. E1 increased cell proliferation on both cellular types either using MTT assay (39 vs 27% a/each C, OB vs VSMC-OB, p