Integrative response of iron cycle proteins by iron excess in neuroblastoma cells

ROQUE MARTA E; GIORGI GISELA; MARÍA FLORENCIA FERNANDEZ DELIAS

MAR DEL PLATA

Congreso; Congreso de la Sociedad Argentina de Investigación Clínica; 2018

SAIC

Many of the known neurodegenerative diseases have been shown to be influenced by changes in brain iron. Unlike other diseases where the association with iron metabolism has been established, the link between iron and neurodegenerative diseases remains unclearThe aim of this study was study the iron effect in neuroblastoma cells(SH-SY5Y) evaluating the expression of iron cycle proteins,ZIP14(Zrt-Irt-like Protein14), DMT1 (divalent metals transporter1) and TfR1(Transferrin receptor1)(importers); H/L-ferritin(storage) and prohepcidin(regulatory protein).The cellular viability was observed by a dose-response curve (neutral red) with low(30-80µM) or high(200-600µM) FAC(ferric ammonium citrate) concentrations. Iron cellular uptake was measured in the culture medium of cells treated with FAC30µM/72hs and 600µM/24hs(FerColor kit). In cells treated with FAC30µM/72hs and/or pretreated with NAC(N-acetylcystein)2mM/12hs the proteins expression and localization were determinated by immunocytochemistry and immunofluorescence. The cellular viability decreased in cells incubated 72hs and 24hs with low or high FAC concentrations(p