Regulación purinérgica de la diferenciación de osteoblastos de rata y efectos de una elevada concentración de glucosa extracelular

GILI V; SANTILLÁN GE; LAIUPPA JA

Congreso; 2° Congreso Conjunto de Osteología, XXXV Reunión Anual AAOMM, XIII Congreso Argentino de Osteoporosis; 2018

Knowledge about osteoblast proliferation and differentiation is essential for development of new anabolic therapies applicable in osteopathies. Ample evidence has shown that GSK3/β-catenin and purinergic signaling have a major role in cellular communication, regulating proliferation and differentiation in many cell types, including osteoblasts.It´s been shown, in vitro, in vivo and in human patients, that chronic hyperglycemia is involved in the pathogenesis of bone loss and high rate of fractures present in diabetes associated osteoporosis. Also, high glucose concentrations reduce ALP activity, osteogenic gene expression and mineralization.The aim of this work was to study the effects of purinergic stimulation and of hyperglycemia on osteoblasts differentiation, in rat calvaria primary culture. Cells were treated with 10 µM UTP, with or without 10 µM Ly294002(Ly) or 8 mM LiCl, or exposed to high glucose concentrations(11, 16.5, 22 and 27.5 mM), for several days, and analyzed for cell viability, by Neutral Red staining, ALP activity, using a commercial kit, and for mineralization, by Alizarin Red staining.Data was analyzed by ANOVA followed by post-hoc analyses with Bonferroni.No change in ALP activity was observed by UTP treatment for 4 or 7 days. However, LiCl augmented(p