BONE VASCULAR ACTIONS OF THE NUTRACEUTICAL GENISTEIN

SANDOVAL MARISA; RASUCHEMBERGER MARIA BELEN; CEPEDA SABRINA; MASSHEIMER VIRGINIA

Buenos Aires

Congreso; REUNION CONJUNTA SOCIEDADES DE BIOCIENCIAS; 2017

SAIC

Previously we reported that the phytoestrogen (PE) Genistein (Gen) prevents the atherosclerotic plaque genesis via estrogen receptor (ER) activation and the inhibition of the cellular/molecular events involved in vascular damage. Indeed, vascular muscle cell transdifferentation into osteoblasts (OB) like cells was also impaired. Here we studied the role of Gen on the bone-vascular axis interaction with focus on OB growth and angiogenesis. To that end, aortic rings (AR), primary cultures of aortic endothelial cells (EC) and calvaria OB isolated from female Wistar rats, exposed to Gen (10nM-1uM) were employed. OB proliferation and differentiation are regulated by several factors such as BMP-2, Runx2 and the bioactive compound NO. We showed that short term exposure of OB to Gen enhanced NO production (33-20% above C, 15-30 min treatment, p