Bone repair: the role of isoflavones on cellular processes involved.

CEPEDA SB; AGOTEGARAY, MA.; MASSHEIMER VL; CAMPELO, AE.; LASALLE V; CUTINI, P.; SANDOVAL MJ

Buenos Aires

Congreso; 34 Reunión Anual AAOMM; 2017

AAOMM

Phytoestrogens (PE) as isoflavone Genistein (Gen) are dietary supplements proposed to reduce the risk of osteoporotic fractures in postmenopausal women. After healing, bone repair depends on angiogenesis that supplies preosteoblasts and factors required for osteoblasts (OB) differentiation, bone formation and mineralization. New capillaries formation involves EC proliferation; migration as well as synthesis of VEGF. Bone remodeling can also be induced by biocompatible silica nanoparticles (NPMsi) capable to generate neo extracellular bone matrix. We previously demonstrated that Gen stimulates bone cells differentiation through its direct action on vascular tissue. In this work we evaluated the role of Gen on the cellular events involved in bone repair. Experimental model: aortic rings (AR); primary cell cultures of aortic endothelial cells (EC) or calvaria OB isolated from female Wistar rats aged 1 to 3 weeks. In vitro treatments with Gen (1 nM-5 ìM) during 24-72 h were performed. PE stimulated EC proliferation in all range of concentrations employed (22-39% above control, p