INBIOSUR   25013
INSTITUTO DE CIENCIAS BIOLOGICAS Y BIOMEDICAS DEL SUR
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Antitumoral and antioxidant activity of the freshwater macroalgae Cladophora surera
Autor/es:
PARODI E; MORELLI S; LEZCANO V; FERNÁNDEZ C
Reunión:
Congreso; 6th Congress of The International Society for Applied Phycology; 2017
Resumen:
Macroalgae are currently being explored as novel and sustainable sources of bioactive compounds for both pharmaceutical and nutraceutical applications arising from its antioxidant, anticancer and antimicrobial activity. In the present study, the antitumoral and antioxidant activities of crude methanolic extract of the freshwater macroalgae Cladophora surera Parodi & Cáceres, harvested from Napostá Creek in Argentina, were investigated in vitro. The antioxidant activity was assessed by DPPH method and polyphenol content using Folin?Ciocalteu phenol reagent. Antitumoral activity was evaluated on the human breast adenocarcinoma cell line MCF?7 by measuring proliferation, migration and cell adhesion. The algae extract (AE) showed high total phenol content (1.62 ± 0.16 µg GAE/mg dry weight algae) and strong scavenging activity on DPPH radical (2.5 ± 0.2 DPPH%/mg dry weight algae).The percentage of alive (unstained) and dead (blue stained) cells was determined by Trypan blue assay. The results indicated that after 48h of treatment the AE significantly inhibits proliferation in a dose-dependent manner, being more effective the highest dose employed (80%±7.6 vs. control); with a concomitant increment in dead cells. However, the colorimetric MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay only showed a significant decrease in cell viability at 100µg/ml AE (20%±3.31 vs. control). Using the wound healing assay we demonstrated that AE inhibits cell migration, with 78.5% and 50.7% of inhibition induced by 10 and 100µg/ml of AE, respectively. Through a cell adhesion assay we found that AE affects considerably the cell adhesion capacity at all doses probed (78.24%±3.6 and 95%±8.36 vs. control, at 10 and 100µg/ml, respectively).Analysis of cell spreading under microscope indicated that cell morphology was also affected by AE treatment. These results indicate that C. surera could be a source of valuable bioactive compounds usable as antitumoral preventive therapy for their effects on the regulation of processes involved in metastasis in cells derived from human mammary cancer (CONICET PIP11220130100070CO; PGI UNS 24/234 and 24/124; PICT 1749).