Comparative actions of 1α,25(OH)2D3-glycosides and synthetic 1α,25(OH)2D3 during myogenesis

IRAZOQUI, P; BUITRAGO, C; AMUSQUIBAR, A; GONZALEZ PARDO, V; DE BOLAND, AR; DE GENARO, P; BACHMANN, H

Cordoba

Congreso; LII Reunión de SAIB; 2016

SAIB

The presence of glycoside derivatives of 1,25(OH)2D3 endows plants to gradual release of the free bioactive form of 1,25(OH)2D3 from its glycoconjugates by endogenous animal glycosidases. This results in increased hormone half-life in blood when purified plant fractions are administered for therapeutic purposes. In this work, we compared the effects of 1,25(OH)2D3-glycosides enriched natural product (Solbone A) with synthetic 1,25(OH)2D3 in skeletal muscle cells C2C12 undergoing differentiation and the role of vitamin D receptor (VDR) on this process. Results showed that Solbone A and 1,25(OH)2D3 (10 nM) increased VDR expression. To explore VDR dependence on C2C12 differentiation, cells were transfected with a shRNA against VDR and then treated with Solbone A or 1,25(OH)2D3 (10 nM, 48 h). qRT-PCR analysis indicated that VDR expression was greatly decreased and microphotographs showed that VDR knockdown cells failed to differentiate. Furthermore, likewise the synthetic hormone, Solbone A increases myogenin, a differentiation marker that was absent in VDR knockdown cells. In conclusion, our results demonstrated that VDR expression is crucial for C2C12 differentiation. Moreover, Solbone A exhibit equal or greater effects on early myoblast differentiation as the synthetic hormone suggesting that plant glycosides could be an effective substitute to promote muscle growth.