INBIOSUR   25013
INSTITUTO DE CIENCIAS BIOLOGICAS Y BIOMEDICAS DEL SUR
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
IRON OVERLOAD INDUCES CELLULAR REDISTRIBUTION OF HEPATIC AND DUODENAL IRON TRANSPORTERS IN MICE.
Autor/es:
FERNANDEZ DELIAS MARIA FLORENCIA; GIORGI GISELA; ROQUE MARTA ELENA; NORMA MARÍA GIUSTO
Lugar:
Mar del Plata
Reunión:
Congreso; . LXI Reunión Cientifica Anual de la Sociedad Argentina de Investigación Clínica. (SAIC) LXIV Reunión Anual de la Sociedad Argentina de Inmunología (SAI), XLVIII Reunión Anual de la Sociedad Argentina de Farmacología Experimental (SAFE), VII Reunión Anua; 2016
Institución organizadora:
SAIC
Resumen:
The liver and duodenum are particularly susceptible to iron-related disorders. These tissues take up plasma iron from transferrin or non-transferrin-bound iron, which appears during iron overload. We assessed the effect of iron status on the levels of the mammalian ZIP14 (Zrt-Irt-like Protein14), divalent metal transporter1 (DMT1) and transferrin receptor1 (TfR1). Objective: The aim of the present study was to determine the effect of iron overload on the expression of iron transporters in the liver and in the duodenum. Materials and Methods: CF1 female mice (25±5g;3 months old) were bred at the animal facility of the UNS. After acclimation, mice were divided in two groups (n=6/group; paired design): 1)Iron adequate (FeA); 2)Iron overload (FeO) Fe-Saccharate (5d/20d.ip; 1,3g/kg). The procedures followed the Guide for the Care and Use of Laboratory Animals of NIH. The protocol was approved by the Committee on Experimental Animal Use and Care of the UNS. Immunohistochemical studies were assessed to determine DMT1, ZIP14, TfR1 localization. Results: Duodenum: In FeA mice, ZIP14 was found mainly in apical membrane of enterocytes. However, slight cytoplasmic ZIP14 expression was seen in FeO. DMT1 expression was detected in the cytoplasm of enterocyte in FeA, however in FeO was manly perinuclear. TfR1 expression detected in perinuclear and basolateral zone of enterocytes was strong in FeA and it was slight in FeO. Liver: ZIP14 and DMT1 expression observed in the cytoplasm of hepatocyte was weak in FeA and it was intense in FeO. Evident TfR1 expression was detected in cellular membrane and in cytoplasm of hepatocytes in FeA, while weak expression was observed in FeO. We detected a significant hemosiderin in Kupffer cells in FeO respect to FeA. Conclusions: The cellular redistribution of duodenal ZIP14, DMT1 y RTf1 and hepatic DMT1 and ZIP14 by iron overload could be explained as a physiological response to decrease the dietary iron uptake and stimulating its hepatic storage.