Bone cellular actions of Genistein (Gen) involves endothelial cells activation via nitric oxide pathway

RAUSCHEMBERGER MB; CRESCITELLI C; MASSHEIMER VL; CEPEDA SB; SANDOVAL MJ

Mar del Plata

Congreso; LX Reunión Científica Anual SAIC (Sociedad Argentina de Investigación Clínica); 2016

SAIC

Previously we showed that, Gen enhances endothelial cells(EC) proliferation and nitric oxide (NO) synthesis, through a mechanismof action that involves the estrogen receptor. In this work, westudied the effect of Gen on bone-vascular axis and, its relationshipwith osteoblastic (OB) differentiation. To that end, primary cultures ofmurine (Wistar rats) endothelial cell (EC) or calvarial preosteoblastcell (OB) were used. OB monolayers were incubated (24 h) with culturemedium obtained from EC (conditioned medium C). Thus, OBproliferation was measured (MTT assay and cell counting). MediumC stimulated OB proliferation (13%, p