INBIOSUR   25013
INSTITUTO DE CIENCIAS BIOLOGICAS Y BIOMEDICAS DEL SUR
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
. ?1,25(OH)2-vitamin D3-rapid actions in rhabdomyosarcoma cells?
Autor/es:
A. PAULA IRAZOQUI; BOLAND R; GUTKIND S; BUITRAGO C
Lugar:
Bariloche
Reunión:
Workshop; III Reunión SISTAM; 2015
Resumen:
We studied for many years the role of vitamin D receptor (VDR) and signal transduction pathways triggered by the steroid hormone 1,25(OH)2-vitamin D3 [1,25D] in avian and mammalian skeletal muscle cells. Recently, we reported VDR and p38 MAPK participation in cell proliferation and differentiation induced by the hormone in the murine skeletal muscle cell line C2C12. There is not information about 1,25D actions in rhabdomyosarcoma (RMS) cells, a soft tissue cancer that affects skeletal muscle predominantly in child and elderly. RMS includes heterogeneous tumours of mesenchymal derivation which are genetically committed to the myogenic lineage, but fail to complete terminal differentiation. In this work, we worked with RD cell line, a embryonal rhabdomyosarcoma cell line. Our data indicated that 1 nM of 1,25D activates ERK1/2 and the p38 MAPK upstream immediately kinases (MKK3/6) rapidly, indicating that the hormone regulates non genomic transduction pathways in these cells. Moreover, the non receptor tyrosine kinase Src was also activated by 1,25D in RD cells. When ERK1/2 were inhibited with PD98059, Src activation was abolished. MKK3/6 activation was also dependent on ERK1/2 activation. Of relevance, 1,25D induces VDR expression in these cells. The results indicate that 1,25D exerts rapid actions in rhabdomyosarcoma cells and open doors to investigate in deep hormone modulation of cancer signaling in these cells.