1a,25(OH)2D3-glycosides from Solanum glaucophyllum promote myogenesis as synthetic 1a,25(OH)2D3 in cultured C2C12 skeletal muscle cells

VALERIA GILI; SILVIA BENASSATI; ANA CAROLINA RONDA; HEINI BACHMAN; RICADO BOLAND

Chicago

Workshop; 17th Workshop on Vitamin D; 2014

Among dietary supplements for broilers, the bioactive derivate of vitamin D3, 1a,25-dihydroxyvitamin D3 (1a,25(OH)2D3) is widely recognized for its improvement of muscle performance. Solanum glaucophyllum has been shown to contain great amounts of 1a,25(OH)2D3 mainly as glycoconjugates. The purpose of this study was to examine the effect of 1a,25(OH)2D3-glycosides from S. glaucophyllum leaf dry powder, known as Solbone (Herbonis AG), compared to synthetic 1a,25(OH)2D3 on myoblast differentiation into myotubes. C2C12 myoblasts were treated with Solbone or free 1a,25(OH)2D3 at the onset of myogenesis. At day 2 of differentiation, 1 nM and 10 nM 1a,25(OH)2D3-glycoside induced a maximum peak expression of the myogenic marker myogenin that dropped rapidly at the third day. The same effects were observed in creatine kinase activity although the peak remained sustained. Altogether, these results suggest that Solbone exert comparable effects on muscle cell differentiation as the animal hormone.