THE BISPHOSPHONATE ALENDRONATE EXERTS A PROTECTIVE ACTION ON VASCULAR RESPONSE INDUCED BY STRESS CONDITIONS.

CUTINI PABLO; VIRGINIA MASSHEIMER

Virtual

Congreso; REUNION DE SOCIEDADES DE BIOCIENCIAS 2021; 2021

SAIC

During menopause women exhibits high prevalence of cardiovascular and bone diseases. Bisphosphonates (BP) represent one of first-line drugs mainly applied for postmenopausal osteoporosis treatment. The aim of this work was to investigate the action of the BP alendronate (ALN) on vascular response to extracellular stress, focusing our attention on the alterations in proliferation and migration cellular patterns and, on the ability to remodel by angiogenesis. Primary cultures of vascular smooth muscle cells (VSMC) and endothelial cells (EC) isolated from murine aorta were used. In order to induce stress conditions, cells were incubated in a pro-osteogenic medium (OM) or exposed to the pro-inflammatory agent LPS. Using the MTT assay, we showed that 1 μM ALN completely or partially suppressed VSMC proliferation induced by LPS (31%, p