INBIOSUR   25013
INSTITUTO DE CIENCIAS BIOLOGICAS Y BIOMEDICAS DEL SUR
Unidad Ejecutora - UE
artículos
Título:
Glycan expression as a tool for a deeper understanding of a reproductive gland in a skate of economic importance
Autor/es:
DIAZ ANDRADE, MC; GALINDEZ, EJ; ACUÑA, FRANCISCO; MOYA, AC; BARBEITO, CLAUDIO
Revista:
JOURNAL OF FISH BIOLOGY
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Lugar: Londres; Año: 2021 vol. 98 p. 548 - 556
ISSN:
0022-1112
Resumen:
Atlantoraja platana is an oviparous skate endemic to the south-west Atlantic Ocean,and is one of the skate species most exploited by local industrial bottom trawl fisheries.Oviparous elasmobranchs encapsulate their eggs in complex egg cases producedby the oviductal gland (OG). This organ is exclusively present in these fishesand comprises four distinct zones: club, baffle, papillary and terminal. The relativesize and structural complexity of these zones correlate with mode of reproduction.Glycans are known to play major roles in reproduction so their distribution in eachzone of the OG could explain the functional multiplicity of the gland in skates, butthis topic has not been previously investigated. In this study, morphological, histochemicaland lectin-histochemical analysis revealed various novel aspects ofA. platana's OG. The club, papillary and terminal zones positively stained for periodicacid Schiff's reagent (PAS) and Alcian Blue (AB), indicating the presence ofneutral and acid mucopolysaccharides. However, the buffle zone was negative forPAS and AB stains, but was positive for all the lectins used. Each zone of the OGhad a characteristic pattern of glycan expression. Finally, we confirmed the presenceof sperm but not sperm storage. This is the first lectin-histochemical study ofthe OG in chondrichtyan fish and it has proven to be an important tool to understandsome of the mechanisms of fertility and reproductive success in economicimportant species such as A. platana.