Morphological and immunochemical characterization of the pollen grains of Chenopodium album L. (Chenopodiaceae) in a temperate urban area in Argentina.

BIANCHIMANO, A.; MURRAY, M. G. *; AZTIRIA, M. E.; MONTES, B.; CALFUÁN, M. L.; PRAT, M. I.

PHYTON - INTERNATIONAL JOURNAL OF EXPERIMENTAL BOTANY BA ARGENTINA

FUNDACION ROMULO RAGGIO

Lugar: Buenos Aires; Año: 2014 vol. 83 p. 9 - 15

0031-9457

Chenopodium album is a very polymorphic, cosmopolitan, annual herb that grows spontaneously in modified soils in wasteland in the outlying districts of the city. In Bahía Blanca the flowering period is mainly during February and March, which coincides with the highest concentrations of this pollen type in the atmosphere of the city. The objective of this study was to characterize the pollen grains of Chenopodium album, both morphologically and immunochemically, that were obtained from three different zones in the urban area of Bahía Blanca. Samples were collected from the three separate zones in the city that were far apart. The structure and morphology of the grains were analyzed using light and electron microscopy. The protein and antigenic profiles were studied with Tricine-SDS-PAGE and western blot with a polyclonal rabbit serum respectively. The morphological analysis showed significant differences in relation to the diameters of the pollen grains in one of the studied areas. Differences in the protein expression were seen for the same area although the antigenic profile was conserved. The variations in the morphology and the protein profile may be caused by the effect of environmental conditions on the pollen and the presence of urban contaminants from vehicular traffic.