Characterization of Molecular Biomarkers associated with Inflammatory Bowel Diseases in the Argentine population

AGUILERA, PABLO; CIMOLAI, MARÍA CECILIA; ROSSO, AYELÉN DAIANA; MILANO, CLAUDIA; BELFORTE, FIORELLA S.; QUESADA, SOFÍA; CEREZO, JIMENA; PENAS-STEINHARDT, ALBERTO

Congreso; LXVI Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica; 2020

Inflammatory bowel disease (IBD) represents a complex chronic disorder comprising two main types: Ulcerative Colitis (UC) and Crohn's Disease (CD). The objective of our study was to identify and characterize molecular biomarkers of IBD in the Argentine population. For this, the circulating expression levels within the Peripheral-Blood-Cell-Fraction (PBCF) and the intestinal expression levels contained in the Fecal-Matter-Cell-Fraction (FMCF) of three miRNAs were studied: hsa-miR-146a-5p, hsa -miR-155-5p and hsa-miR-223-3p. 57 individuals were recruited from the Posadas Hospital Gastroenterology Service, from which 51 blood samples (13 CT, 20 CU and 18 CD) and 38 stool samples (12 controls, 15 CU and 11 CD) were obtained. Total RNA was extracted and retro-transcribed using the MMLV reverse enzyme and Stem Loop Primers designed for this purpose. The quantification was carried out by qPCR by means of Absolute Quantification by Standard Curve using synthetic plasmids. The data obtained were statistically analyzed with the GraphPad Prism 6.01 program using non-parametric tests. The results (p