EFFECT OF ANTIOXIDANT HORTICULTURAL EXTRACTS ON ACETOMINOPHEN HEPATOTOXICITY IN HEP-G2

ORELLANO M; GUERRA LILIANA N; VICENTE G; DE MARZI M

Congreso; ANNUAL MEETING OF BIOSCIENCE SOCIETIES 2020 - LXV ANNUAL MEETING OF SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA (SAIC); 2020

Sociedad Argentina de Investigaciones Clinicas

We have demonstrated that antioxidant N-Acetylcysteine (NAC)inhibits lipids accumulation in 3T3-L1 adipocytes (AD) (Soto et al.2017 and 2020). NAC is also an antidote for acetaminophen (AP)hepatotoxicity in vivo, decreasing to 50% the levels of hepatic aspartateaminotransferase (AST) and alanine aminotransferase(ALT) activities after 24 h NAC treatment. Our aim is to evaluate horticulture extracts (HE) with antioxidant capacity (AO) as hepaticprotectors and compare their effects with NAC. Methods: Assayswere conducted with HE from Citrus sinensis (orange), Silybummarianum (thistle), Quassia amara (quassia), Baccharis articulata(carqueja), Peumus boldus (boldo), Cynara scolymus (artichoke),Verbena bonariensis (verbena), Schinopsis balansae (quebracho,rich in polyphenols) and Ribes nigrum (cassis). We determined AOby DPPH assay. Hep-G2 cells were treated with 15 mM AP and HEfor 24h. After these treatments, we evaluated cytotoxicity by MTTassay, ALT and AST activities. In HE-treated AD, we assessed cholesterol(Chol) and triglyceride (Tg) levels. Results: The highest HEAO/mg were 88 + 5% (orange, OE), 74 + 1% (carqueja, CE), 70+ 2% (quebracho, QE) and 68 + 4% (cassis, CsE). CsE was toxicto Hep-G2 cells, but CsE treatment on AD resulted in a significantdecrease in Chol (by 31%) and Tg (by 62%) levels, without toxiceffects. AP treatment was toxic to Hep-G2 cells and increased by 3folds AST and ALT activities compared to non-treated control cells(p