APPROACH TO DELIVER N-ACETYLCYSTEINE WITH NANOPARTICLES INTO CELLS

LAURA MONTALDO; ROMINA MITAROTONDA; LILIANA NOEMÍ GUERRA; EVELYN FRONTERA; MAURICIO DE MARZI

Ferrara

Congreso; Society for Free Radical Research Europe; 2019

Society for Free Radical Research Europe

In our laboratory we evaluated N-Acetylcysteine (NAC) effects by its addition to cell culture media. We demonstrated that NAC inhibits adipogenic differentiation of 3T3-L1 preadipocytes (Soto et al, 2017). Here, we performed treatments with 0.01 to 5 mM NAC, on 3T3-L1 adipocytes (AC). NAC cellular uptake, in 5mM NAC treated 3T3-L1 adipocytes (ACN-5), was only 17.4% of total NAC added. Highest NAC dose decreased total cholesterol content (Chol) (1.61 + 0.50 g Chol / g protein [ACN-5] vs 5.73 + 0.02 g Chol/ g protein [AC], p < 0.05). We evaluated cytotoxicity by colorimetric assay using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; none dose of NAC was cytotoxic (OD: 0.85 + 0.02 [ACN-5] vs 0.81 + 0.08 [AC], no significant difference). We developed spherical porous silica nanoparticles (20 + 4.5 nm), in which we encapsulated 5mM NAC (NPsSiO2-NAC), and evaluated their cytotoxicity. Samples of 0.05mg/mL showed 42.3 % cytotoxicity (OD: 0.41+0.04 [NPsSiO2-NAC] vs 0.71+0.04 [nontoxic control], p < 0.01); with a cover of bovine sera albumin (8nm) we obtained nontoxic NAC-nanoparticles (OD: 0.91+0.09 [NPsSiO2-NAC] vs 0.90+0.08 [nontoxic control], no significant difference). NAC release was completed within 28 hours. This tool for NAC delivery could increase NAC cellular uptake and its effect on lipid content.