INEDES   24797
INSTITUTO DE ECOLOGIA Y DESARROLLO SUSTENTABLE
Unidad Ejecutora - UE
artículos
Título:
Histological, genotoxic, and biochemical effects on Cnesterodon decemmaculatus (Jenyns 1842) (Cyprinodontiformes, Poeciliidade): early response bioassays to assess the impact of receiving waters
Autor/es:
CASTAÑÉ, PATRICIA M.; FERRARI L; OSSANA, N A; TRIPOLI L; BAODOU F G; SOLONESKI S
Revista:
Jounal of toxicology
Editorial:
Hindawi
Referencias:
Lugar: El Cairo; Año: 2019
ISSN:
1687-8191
Resumen:
In the present study, the toxicity of receiving waters from a highly polluted urban watercourse, the Reconquista River, Argentina, collected at a dam in the upstream part of the river was evaluated. Cnesterodon decemmaculatus, a widely distributed fish species in Pampasic rivers proposed for use in ecotoxicological evaluations, was used as a test organism. A 96-h acute toxicity bioassay with river water quality has been characterized as moderately contaminated was performed. The treatment groups were: 1) whole surface river water; 2) whole surface river water with 2 mg Cd/L added as a simulated metal contaminant pulse; 3) a negative control using reconstituted moderately hard water (MHW); 4) a metal positive control, MHW + 2 mg Cd/L; and 5) a positive genotoxicity control, MHW + 5 mg Cyclophosphamide/L (CP). The condition factor rate, micronuclei frequency and comet assay from peripherical blood, structural changes of the gill arrangement by scanning electron microscope analysis, histopathological changes in the liver and the glutathione-S-transferase, catalase, superoxide dismutase, glutathione and protein content from the body midsection (viscera) were evaluated. According to our results, for short term exposure, SEM analyses of gills and liver histopathological analyses could be useful tools for the evaluation of target organ damage as well as comet assays for DNA damage. We propose that the 96-h laboratory bioassay protocol described is useful for monitoring the deterioration of water quality employing the teleost C. decemmaculatus and that the microscope analysis of gills and liver as well as the comet assay methodology could be sensitive endpoint indicators.